In 2004, Apple Tree opened the Madelia Center in the local hospital to help meet dental access needs among a low-income Hispanic population in southwestern Minnesota.

In 2009, Apple Tree opened the Fergus Falls Center to help provide dental services and fill longstanding gaps in the region.

In 2015, Apple Tree opened the San Mateo Center in California, creating a dental program to meet growing gaps in the availability of geriatric and special care dentistry services in the area. This program was established through an affiliation with Sonrisas Community Dental care, which was founded in 2001.

In 2017, Apple Tree transitioned the management of the San Mateo and Sonrisas Centers to local leaders. The new organization managing these Centers is now called Sonrisas Dental Health.

In 2018, Apple Tree transitioned the Little Falls Outreach Clinic to the Little Falls Center for Dental Health, open five days a week with a full-time dentist.

In 2020, in the Mayo Clinic Health System in Fairmont, Apple Tree opened the Fairmont Center for Dental Health with mobile equipment. In April 2021, the Fairmont Center moved into a refurbished section of the same building and opened with a full team.

In the spring of 2023, Apple Tree opened the new-and-improved Little Falls Center within St. Gabriel's Hospital. The project was made possible with funding and support from various groups.